Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
نویسندگان
چکیده
منابع مشابه
Improved quantitative real-time RT-PCR for expression profiling of individual cells.
The real-time quantitative polymerase chain reaction (rtqPCR) has overcome the limitations of conventional, time-consuming quantitative PCR strategies and is maturing into a routine tool to quantify gene expression levels, following reverse transcription (RT) of mRNA into complementary DNA (cDNA). Expression profiling with single-cell resolution is highly desirable, in particular for complex ti...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2014
ISSN: 1940-087X
DOI: 10.3791/51408